Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Diagnosis > Laboratory Techniques and Procedures > Immunologic Tests > Fluorescent Antibody Technique Terms and Definitions




Fluorescent Antibody Technique

Medical Definition: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Guide Notes: NIM; GEN or unspecified; prefer specifics; DF: FANA TEST
Also Called: Fluorescent Protein Tracing,Immunofluorescence
Fluorescent Antibody Technique Categories.
Canadian Medical Guide Definition Fluorescent Antibody Technique, Direct - A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Canadian Medical Guide Definition Fluorescent Antibody Technique, Indirect - A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Canadian Medical Guide Definition Fluoroimmunoassay - The use of fluorescence spectrometry to obtain quantitative results for the FLUORESCENT ANTIBODY TECHNIQUE. One advantage over the other methods (e.g., radioimmunoassay) is its extreme sensitivity, with a detection limit on the order of tenths of microgram/liter.

Fluorescent Antibody Technique Medical Definitions and Terms

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