Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Microscopy > Microscopy, Fluorescence Terms and Definitions




Microscopy, Fluorescence

Medical Definition: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Guide Notes: NIM
Also Called: Immunofluorescence Microscopy
Microscopy, Fluorescence Categories.
Canadian Medical Guide Definition Microscopy, Fluorescence, Multiphoton - Flurorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and photoxicity to the area being imaged.

Microscopy, Fluorescence Medical Definitions and Terms

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