Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Microscopy > Microscopy, Fluorescence > Microscopy, Fluorescence, Multiphoton Terms and Definitions




Microscopy, Fluorescence, Multiphoton

Medical Definition: Flurorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and photoxicity to the area being imaged.
Previously Indexed: Microscopy, Confocal (1997-2002),Microscopy, Fluorescence (1996-2002)

Microscopy, Fluorescence, Multiphoton Medical Definitions and Terms

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