Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Clinical Laboratory Techniques > Cytological Techniques > Histocytological Preparation Techniques Terms and Definitions

Histocytological Preparation Techniques

Medical Definition:	Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.
Guide Notes:	DF: HISTOCYTOL PREP TECHNIQUES

Histocytological Preparation Techniques Categories.

	Microtomy - The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
	Replica Techniques - Methods of preparing tissue specimens for visualization using an electron microscope, usually a scanning electron microscope. The methods involve the creation of exact copies of the specimens by making a mold or cast (i.e., replica) of the specimen.
	Staining and Labeling - The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
	Tissue Embedding - The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.

Histocytological Preparation Techniques Medical Definitions and Terms

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