Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Centrifugation > Ultracentrifugation > Centrifugation, Density Gradient Terms and Definitions




Centrifugation, Density Gradient

Medical Definition: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Guide Notes: a type of ultracentrif; NIM; index sucrose density gradient centrif here but ignore SUCROSE
Centrifugation, Density Gradient Categories.
Canadian Medical Guide Definition Centrifugation, Isopycnic - A technique used to separate particles according to their densities in a continuous density gradient. The sample is usually mixed with a solution of known gradient materials and subjected to centrifugation. Each particle sediments to the position at which the gradient density is equal to its own. The range of the density gradient is usually greater than that of the sample particles. It is used in purifying biological materials such as proteins, nucleic acids, organelles, and cell types.
Canadian Medical Guide Definition Centrifugation, Zonal - Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Centrifugation, Density Gradient Medical Definitions and Terms

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