Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Chemistry, Analytical > Photometry > Spectrophotometry Terms and Definitions

Spectrophotometry

Medical Definition:	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
Guide Notes:	a type of spectrum anal; NIM

Spectrophotometry Categories.

	Microspectrophotometry - Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.
	Spectrophotometry, Atomic - Spectrophotometric techniques in which the samples are examined in the form of atoms based on their unique energy levels for ELECTRONS. They are used to analyze TRACE ELEMENTS, such as ALUMINUM; ARSENIC; BERYLLIUM; CALCIUM; COPPER; IRON; LEAD; and LITHIUM.
	Spectrophotometry, Infrared - Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
	Spectrophotometry, Ultraviolet - Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Spectrophotometry Medical Definitions and Terms

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