Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Genetic Techniques > Nucleic Acid Amplification Techniques > Polymerase Chain Reaction Terms and Definitions




Polymerase Chain Reaction

Medical Definition: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Also Called: Anchored PCR,Inverse PCR,Nested Polymerase Chain Reaction
Previously Indexed: Gene Amplification (1988-1989)
Polymerase Chain Reaction Categories.
Canadian Medical Guide Definition Reverse Transcriptase Polymerase Chain Reaction - A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

Polymerase Chain Reaction Medical Definitions and Terms

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