Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Genetic Techniques > Molecular Probe Techniques Terms and Definitions




Molecular Probe Techniques

Medical Definition: The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.
Guide Notes: specific probes are indented under MOLECULAR PROBES in Cat D26; DF: MOL PROBE TECHNIQUES
Molecular Probe Techniques Categories.
Canadian Medical Guide Definition Biotinylation - Incorporation of biotinyl groups into molecules.
Canadian Medical Guide Definition Blotting, Southwestern - A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies.
Canadian Medical Guide Definition Branched DNA Signal Amplification Assay - A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection.
Canadian Medical Guide Definition Oligonucleotide Array Sequence Analysis - Hybridization of a nucleic acid sample to a very large set of oligonucleotide probes, which are attached to a solid support, to determine sequence or to detect variations in a gene sequence or expression or for gene mapping.
Canadian Medical Guide Definition Protein Array Analysis - Ligand-binding assays that measure protein-protein, protein-small molecule or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on the solid support, to measure the presence or interaction of target molecules in the sample.
Canadian Medical Guide Definition Random Amplified Polymorphic DNA Technique - Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.

Molecular Probe Techniques Medical Definitions and Terms

CANADIAN MEDICAL GUIDE

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