Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Genetic Techniques > In Situ Nick-End Labeling Terms and Definitions




In Situ Nick-End Labeling

Medical Definition: An in situ method for detecting areas of DNA which are nicked during apoptosis. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA fragmentation.
Previously Indexed: Apoptosis (1994-1998)

In Situ Nick-End Labeling Medical Definitions and Terms

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