Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Genetic Techniques > Cloning, Molecular Terms and Definitions

Cloning, Molecular

Medical Definition:	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Guide Notes:	differentiate from CLONE CELLS and CLONING, ORGANISM; "clonal analysis": index under CLONE CELLS; DF: CLONING MOL or MOL CLONING
Previously Indexed:	DNA (1966-1976),DNA, Recombinant (1977-1979),Plasmids (1975-1979)

Cloning, Molecular Categories.

	Gene Fusion - Fusion of structural genes to analyze protein behavior or fusion of regulatory sequences with structural genes to determine mechanisms of regulation.
	Two-Hybrid System Techniques - Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules.

Cloning, Molecular Medical Definitions and Terms

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