Canadian Medical Guide > Analytical, Diagnostic and Therapeutic Techniques and Equipment > Investigative Techniques > Genetic Techniques > Cloning, Molecular Terms and Definitions
Cloning, Molecular
Medical Definition: | The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. |
Guide Notes: | differentiate from CLONE CELLS and CLONING, ORGANISM; "clonal analysis": index under CLONE CELLS; DF: CLONING MOL or MOL CLONING |
Previously Indexed: | DNA (1966-1976),DNA, Recombinant (1977-1979),Plasmids (1975-1979) |
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Gene Fusion - Fusion of structural genes to analyze protein behavior or fusion of regulatory sequences with structural genes to determine mechanisms of regulation. |
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Two-Hybrid System Techniques - Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules. |
Cloning, Molecular Medical Definitions and Terms
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