In Situ Nick-End Labeling
| Medical Definition: |
An in situ method for detecting areas of DNA which are nicked during apoptosis. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA fragmentation. |
| Previously Indexed: |
Apoptosis (1994-1998) |
In Situ Nick-End Labeling Medical Definitions and Terms
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